BÜHLMANN Quantum Blue® Adalimumab is an in vitro diagnostic lateral flow immunoassay for the quantitative determination of trough levels of adalimumab in serum samples.
The assay serves as an aid to therapeutic drug monitoring in patients with inflammatory bowel disease (IBD) under adalimumab therapy, in conjunction with other clinical and laboratory findings. Quantum Blue Adalimumab is combined with the Quantum Blue Reader. The test is designed for the selective measurement of adalimumab by a sandwich immunoassay.
Recombinant tumor necrosis factor alpha (TNFα) is conjugated to gold colloids. On the test cassette the gold conjugate is released from a pad into the reaction system as the sample is applied. Adalimumab present in the sample will bind to the gold conjugate. A monoclonal antibody, highly specific for adalimumab, is immobilised on the analytical membrane and will capture the complex of gold conjugate and the adalimumab analyte, resulting in a colouring of the Test Line (T). The remaining free TNFα-gold conjugate will bind to the Control Line (C). The signal intensities of the Test Line (T) and the Control Line (C) are measured quantitatively by the Quantum Blue Reader.