Calprotectin Extraction Methods

Weighing

Weighing the sample is still considered the Gold-Standard sample extraction method.

Step 1 Step 2 Step 3 Step 4
Pre-weigh empty tube and inoculation loop and tare to zero. Add 50 – 100mg faeces Add 49 volumes of 1x B-CAL-EX Close tube and vortex vigorously for 30 min (highest speed).
Step 5 Step 6 Step 7 Step 8
Transfer 1.5ml into a fresh tube. Centrifuge for 5 min at 10,000 x g. Transfer supernatant into a fresh tube Continue immediately to ELISA procedure, or store at >-20°C
See ELISA Product Details

 

CALEX® Cap (BÜHLMANN)

CALEX® Cap is a new stool extraction device containing a measured amount of BÜHLMANN calprotectin extraction buffer. It is for exclusive use with all BÜHLMANN calprotectin assays and has very high correlation to the gold standard weighing extraction method.

It simplifies sample handling eliminating the need for weighing, pipetting or decanting and enables direct loading of the extraction device onto ELISA processors.

Step 1 Step 2 Step 3 Step 4 Step 5
calex-cap-150x150-1 calex-cap-150x150-2 calex-cap-150x150-3 calex-cap-150x150-4 calex-cap-150x150-5
Hold the CALEX Cap device upright and remove the white sampling pin by simultaneously turning and pulling it upwards. Do not unscrew the blue cap! Dip the sampling pin with the grooves into the stool turn the pin and remove it afterwards. Repeat the procedure at 3-5 different positions of the sample in order to fill the grooves completely.Note: Excess stool is stripped off in the funnel during re-introduction of the sampling pin into the CALEX body Re-introduce the sampling pin into the CALEX body and push it back into the final locking position (you feel and hear two clicks). Vortex the CALEX Cap device with the white head down vigorously on a vortex mixer for 30 sec and allow it to stand for 10 min on the white head. If the stool does not completely come off the grooves, repeat the procedure until the grooves are completely free of stool. That is easily monitored by checking that the grooves are empty before you proceed with step 5. Unscrew the blue cap and take the appropriate sample volume for the analysis; continue with the respective Calprotectin assay.Note: Tightly recap the CALEX Cap device after usage

See CALEX Cap Product Details View CALEX Cap Instructions for Use (IFU)

 

Smart-Prep (Roche)

Step 1 Step 2 Step 3 Step 4 Step 5
Carefully press the faeces sample into the hollow cavity in the base cap and level off the surface. Firmly press the tube onto the base cap and detach the tag. Remove the stopper cap and add 4ml of extraction buffer (BCAL-EX). Carefully re-cap the tube and homogenize the sample for 1 min using a vibration mixer (e.g. Vortex mixer) until no large particles can be seen. For further sample processing and dilution refer to the instruction for use of the respective Calprotectin kit.

See Smart-Prep Product Details Roche vs Weighing Extraction Correlation Watch Extraction Video

 

Quick-Prep (Schebo)

Step 1 Step 2 Step 3 Step 4 Step 5
Turn the dosing tip counter-clockwise and remove it by pulling it upwards. Insert the dosing tip into the fecal sample (depth appr. 2 cm). Repeat sampling 3-5 times in order to fill the grooves completely. Insert the dosing tip back into the tube and firmly close it by turning the dosing tip clockwise. Homogenize the sample by vortexing for 30 sec., let it stand for 10 min. and vortex again for 30 sec. using a vibration mixer. Repeat this step until the faeces has completely been removed from the dosing tip. Turn the cone containing the dosing tip counter-clockwise and remove by pulling it upwards. Extract processing and dilution for the Calprotectin determination are described in the instruction for use of the respective kit.

See Quick-Prep Product Details Watch Extraction Video