Weighing the sample is still considered the Gold-Standard sample extraction method.
|Step 1||Step 2||Step 3||Step 4|
|Pre-weigh empty tube and inoculation loop and tare to zero.||Add 50 – 100mg faeces||Add 49 volumes of 1x B-CAL-EX||Close tube and vortex vigorously for 30 min (highest speed).|
|Step 5||Step 6||Step 7||Step 8|
|Transfer 1.5ml into a fresh tube.||Centrifuge for 5 min at 3,000 x g.||Transfer supernatant into a fresh tube||Continue immediately to ELISA procedure, or store at >-20°C|