Quantitative High Throughput Test

EK-CAL Calprotectin ELISA
EK-CAL Calprotectin ELISA

The BÜHLMANN fCAL® ELISA is a microtitre based assay kit for determination of calprotectin in faecal samples. All reagents, standards and controls are provided in one kit and are ready for use. There is no requirement for reconstituting any component, apart from the wash buffer. This minimises any errors and ensures a long shelf life. The ELISA can be performed manually or on a range of automated processors to reduce hands–on time in higher throughput environments.

Two different protocols are available in one kit in order to ensure that the range for IBD and IBS differentiation and therapy monitoring is covered. The ranges are 10-600µg/g or 30-1800µg/g. A consistent cut-off of 80µg/g is used throughout the BÜHLMANN Calprotectin range.

A simple extraction procedure for Calprotectin is available using either the CALEX Cap or Roche extraction devices. Click here to find out more about these extraction devices.

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Calprotectin ELISA
Product Code Description Pack Size
EK-CAL fCAL ELISA single plate 96 wells
EK-CAL2 fCAL ELISA two plates 192 wells
EK-CAL2-WEX fCAL ELISA two plates without buffer 192 wells
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For higher throughput calprotectin testing requirements the market-leading BÜHLMANN fCAL® ELISA provides an excellent laboratory solution.

It can be easily automated on many commercially available ELISA processors. There are CE marked protocols available for the Dynex DS2 and DSx plus the Triturus instrument.

Alpha Laboratories Ltd can support your automation requirements by supplying the Dynex DS2 for capital purchase or on a reagent rental scheme.

The kit contains all reagents, standards and controls provided ready for use. With no requirement for reconstituting components (except wash buffer), errors are minimised and a long shelf life is achieved.

  • Easy-to-use ELISA kit (total assay time is 75 minutes)
  • All reagents are ready to use*
  • Kit includes calibrators and controls
  • Analytical Sensitivity: <10µg/g Calprotectin in faecal sample
  • Measurable range 30-1800µg/g or 10-600µg/g**

* except wash buffer
** depending on which protocol is used

Studying Calprotectin False at Luton and Dunstable University Hospital
False Positive Faecal Calprotectin?
The Camera Never Lies

Louise Asser, Specialist Biomedical Scientist at Luton and Dunstable University Hospital

Faecal calprotectin—ready for prime time?

Introduction Calprotectin is a heterodimer of calcium-binding proteins which belongs to

The Pitfalls of Diagnosing IBS without Checking Calprotectin

In this case study, Arne Roseth reflects on a case last

Gold Star for Service

Successful laboratory assays are usually dependent on the alignment of many