Speed, Quality and Flexibility in Calprotectin Quantification

fCAL turbo calprotectin turbidimetric assay
fCAL turbo calprotectin turbidimetric assay

BÜHLMANN fCAL® turbo is a random access calprotectin assay for use on major clinical chemistry platforms.

Utilising immunoturbidimetry it  provides a very rapid and flexible solution for high throughput applications in the routine laboratory. fCAL turbo allows short turnaround time from stool sample to reportable result.

Speed: the fastest calprotectin test in the market.

  • Results in 10 minutes.
  • Broad measurable range 20 to 8000 µg/g calprotectin reduces the amount of retesting down to <5%.

Quality: The assay is standardised against the BÜHLMANN fCAL® ELISA, the same cut-offs can be applied. Particle-enhanced turbidimetric immunoassay (PETIA) further increases the precision and reproducibility of calprotectin quantification.

Flexibility: Immunoturbidimetric assays can be applied on any open clinical chemistry platform. This allows random access workflow which can be directly integrated into the existing laboratory infrastructure.

Use with CALEX® Cap device for easy and efficient stool extraction or CALEX® Track for complete automation

Available Application Protocols:

  • Abbott: Architect c-series, Alinity
  • Beckman: AU Series (AU400, AU480, AU640, AU680, AU2700, AU5400, AU5800), DxC 600/800
  • Mindray: BS-380, BS-200E, BS-400
  • Ortho: Vitros 5600
  • Roche: Cobas c111, c501, c502, c503, c701, c702
  • Siemans: Advia 1650/1800, Advia 2400, Advia XPT, Dimension Vista500/1500*, Atellica
  • Thermo Fisher: Konelab30i, Indiko
  • The Binding Site: Optilite
  • Horiba: ABX Pentra 400
  • IDS: iSYS
  • BioSystems: BA200

Mark Busbridge, Lead Biomedical Scientist, Charing Cross Hospital reports his experience on moving to the BÜHLMANN fCAL® turbo calprotectin assay:

“We have been analysing faecal samples for calprotectin in-house since 2011. As with most large multi-site NHS Trusts we have seen a significant increase in calprotectin workload during this period, with the Trust currently processing 70-80 samples per day.

Our principle methods have been the Phadia EliA I/II on the ImmunoCAP 100/250 analysers. However, with the increasing sample workload we were struggling to maintain the TAT with our current method, due to prolonged assay time (2 hours), significant ImmunoCAP 250 maintenance downtime and the lengthy sample extraction process.

So in 2018 we began investigating alternative methods. The BÜHLMANN fCAL® turbo calprotectin assay was selected, as this can be run on one of our principle analysers (Abbott Architect C8000). The BÜHLMANN CALEX extraction device process is simple and straightforward for staff to follow, resulting in a much improved workflow compared to the previous method, allowing more staff flexibility in this section.

Since going ‘live’ with the method in late 2019, we have noted improved precision in our inter batch re-extracted patient QC samples, good sample linearity and excellent repeatability and we are currently observing good agreement with our EQA method mean group.”

Key Literature


Complete Calprotectin Automation with Track CALEX® from BÜHLMANN

CALEX® Cap on clinical chemistry analyser


Particle-enhanced turbidimetric immunoassay (PETIA)

Sample Type Faecal extracts
Kit Format 2 reagents; wedge bottles for direct loading on many platforms

Reagents last for ~200 tests; platform dependent

All assay components are also available individually

Sample Preparation

CALEX® Cap extracts can be used directly without additional dilution

Reagent on Board  Stable for at least 60 days
Calibration  0-2000 μg/g
Measuring Range 20-8000 μg/g
Sample Volume ~10 µl Extract (1:500)
Time to Result ~10 minutes


On many major platforms

Liquid Elastase Samples – To Test, or Not to Test? That is The Question…

Alison Jones, Consultant Clinical Biochemist, York and Scarborough NHS Foundation Trust

Switching things up… Transitioning the BÜHLMANN fCAL® turbo for Calprotectin Testing

From the Siemens Advia 2400 to the Atellica Analyser Georgette Glover,

Switching from Phadia to BÜHLMANN for a More Efficient Calprotectin Service

The Newcastle upon Tyne Hospitals Foundation Trust is one of the

Experience of the BÜHLMANN fCAL® turbo on DxC 800, Beckman Coulter

A Report of Martina Kobelt* and Karin Jung**, Zentrum für Labormedizin,

Unilabs Portugal using BÜHLMANN fCAL® turbo in routine

Unilabs Portugal, a private European lab chain, made a comprehensive evaluation

From BÜHLMANN Quantum Blue® to fCAL turbo®

Follow the Evolution of Calprotectin Testing at James Cook University Hospital This

Calprotectin and Clinical Correlation

Mary O’Connell née Deasy, Biochemistry Department, Mercy University Hospital, Cork In issue

fCAL turbo vs. IBDoc
Calprotectin Home vs. Laboratory Test

Mary O’Connell née Deasy, Biochemistry Department, Mercy University Hospital, Cork, Ireland

Mary OConnell
Bringing Calprotectin Testing In-house on the Abbott Architect C8000

Mary O’Connell née Deasy, Biochemistry Department Mercy University Hospital Cork, Ireland

Rosie Forster, Queen Elizabeth Hospital Birmingham, with the CALEX and Roche cobas c702
Introducing in-house Calprotectin Testing Directly on the Roche cobas® c702

Turbo Charged from the Start Rosie Forster and Karen Smith, Clinical

Buy Online

fCAL® turbo
Product Code Description Pack Size*
KK-CAL fCAL turbo kit (reagents, controls and
B-KCAL-RSET fCAL turbo reagents ~200
B-KCAL-CASET fCAL turbo calibrators 6 levels, 1ml each
B-KCAL-CONSET fCAL turbo controls 3 x 2 levels, 1ml each
B-CAL-EX12 Extraction buffer 12 x 125ml

*Will vary depending on analyser

Buy fCAL turbo online