Speed, Quality and Flexibility in Calprotectin Quantification

fCAL turbo calprotectin turbidimetric assay
fCAL turbo calprotectin turbidimetric assay

BÜHLMANN fCAL® turbo is a random access calprotectin assay for use on major clinical chemistry platforms.

Utilising immunoturbidimetry it  provides a very rapid and flexible solution for high throughput applications in the routine laboratory. fCAL turbo allows short turnaround time from stool sample to reportable result.

Speed: the fastest calprotectin test in the market.

  • Results in 10 minutes.
  • Broad measurable range 20 to 8000 µg/g calprotectin reduces the amount of retesting down to <5%.

Quality: The assay is standardised against the BÜHLMANN fCAL® ELISA, the same cut-offs can be applied. Particle-enhanced turbidimetric immunoassay (PETIA) further increases the precision and reproducibility of calprotectin quantification.

Flexibility: Immunoturbidimetric assays can be applied on any open clinical chemistry platform. This allows random access workflow which can be directly integrated into the existing laboratory infrastructure.

Use with CALEX® Cap device for easy and efficient stool extraction or CALEX® Track for complete automation

Available Application Protocols:

  • Abbott: Architect c-series
  • Beckman: AU Series (AU400, AU480, AU640, AU680, AU2700, AU5400, AU5800), DxC 600/800
  • Mindray: BS-380, BS-200E, BS-400
  • Ortho: Vitros 5600
  • Roche: Cobas c111, c501, c502, c701, c702
  • Siemans: Advia 1650/1800, Advia 2400, Advia XPT, Dimension Vista500/1500*
  • Thermo Fisher: Konelab30i, Indiko
  • The Binding Site: Optilite
  • Horiba: ABX Pentra 400
  • IDS: iSYS

Key Literature

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fCAL® turbo
Product Code Description Pack Size*
KK-CAL fCAL turbo kit (reagents, controls and
B-KCAL-RSET fCAL turbo reagents ~200
B-KCAL-CASET fCAL turbo calibrators 6 levels, 1ml each
B-KCAL-CONSET fCAL turbo controls 3 x 2 levels, 1ml each
B-CAL-EX12 Extraction buffer 12 x 125ml

*Will vary depending on analyser

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Particle-enhanced turbidimetric immunoassay (PETIA)

Sample Type Faecal extracts
Kit Format

2 reagents; wedge bottles for direct loading on many platforms

Reagents last for ~200 tests; platform dependent

All assay components are also available individually

Sample Preparation

CALEX® Cap extracts can be used directly without additional dilution

Reagent on Board  Stable for at least 60 days
Calibration  0-2000 μg/g
Measuring Range 20-8000 μg/g
Sample Volume ~10 µl Extract (1:500)
Time to Result ~10 minutes


On many major platforms

Loading fCAL turbo faecal calprotectin tests onto the cobas 6000
On Board with fCAL® turbo at Cumberland Infirmary

Pamela Bowe, Team Manager in the Department of Biochemistry at the

fCAL turbo faecal calprotectin assay
BÜHLMANN fCAL® turbo Faecal Calprotectin Assay Proves Consistent between Multiple Analysers

There has been much interest in the BÜHLMANN fCAL turbo faecal calprotectin

BÜHLMANN fCAL turbo Evaluation
Bioanalytica Lucerne 2: BÜHLMANN fCAL® turbo evaluation on the Roche cobas® c501

Interview with Yvonne Schallberger, Bioanalytica Lucerne 2. BÜHLMANN fCAL turbo Evaluation

Evaluating fCAL turbo - Beckman UniCel DxC 600
Children’s Hospital of Zurich introduces fCAL turbo on the Beckman UniCel DxC 600

Interview with Mara Barbieru Deputy Senior Biomedical Analyst, Department of Clinical Chemistry and

Anita-Grunauer evaluates fCAL turbo on Roche c501
City Hospital Triemli Optimizes Laboratory Workflow For Calprotectin Testing

Interview with Anita Grünauer (BMA), Dr. Jasmin Barman-Aksözen (Head of Department

Rosie Forster, Queen Elizabeth Hospital Birmingham, with the CALEX and Roche cobas c702
Introducing in-house Calprotectin Testing Directly on the Roche cobas® c702

Turbo Charged from the Start Rosie Forster and Karen Smith, Clinical

Mary OConnell
Bringing Calprotectin Testing In-house on the Abbott Architect C8000

Mary O’Connell née Deasy, Biochemistry Department Mercy University Hospital Cork, Ireland

fCAL turbo vs. IBDoc
Calprotectin Home vs. Laboratory Test

Mary O’Connell née Deasy, Biochemistry Department, Mercy University Hospital, Cork, Ireland

Calprotectin and Clinical Correlation

Mary O’Connell née Deasy, Biochemistry Department, Mercy University Hospital, Cork In issue