Calprotectin Extraction

Preparation of a simple internal quality control for Faecal Calprotectin, from Jane French, Consultant Clinical Scientist, UK NEQAS.

“As Programme Director of the UK NEQAS for Faecal Markers, I am often asked if I can recommend a suitable internal quality control (IQC) material for Faecal Calprotectin. Until quite recently, there has been no commercially-produced IQC available but all is not lost – with a bit of know-how, it is perfectly possible to make your own bespoke IQC, tailored to your exact requirements.”

The manual weighing method with a minimal amount of 50-100 μg fecal sample extracted in 50 volumes of extraction buffer was established in the early 2000 and guarantees the best possible quantification of fCAL. The method is regarded as the reference method for fCAL quantification. New devices simplifying the time consuming reference method have been introduced by many different immunoassay manufacturers. The new CALEX® Cap extraction device from BÜHLMANN was validated in this study.

Calex® extraction devices compare well to extraction via manual weighing. Calex® also demonstrated similar imprecision and accuracy to manual extraction.
The main benefits of Calex®, speed and ease of use, will enable higher throughput and will allow the service to cope with future increases in demand.
The devices are also more hygienic and the significant reduction in staff time and other reagents/consumables will no doubt result in an overall cost saving.

The stool sampling using CALEX® Cap is very reproducible, easier and more hygienic than manual weighing. The extraction of
calprotectin from stool samples is as reliable as with conventional methods. CALEX® Cap represents a fully quantitative “all-in-one” device.

Overall the Eurospital extraction devices had more disadvantages than the BÜHLMANN Calex tubes, being more laborious with an acceptable risk of splashing the extract

fCAL turbo is very adaptable to the Abbott Architect C8000 analyser. The CALEX ® cap extraction devices are easy to use and practical. Inter-assay variability demonstrated, and recommends users to participate in the UK NEQAS Scheme . No interference to any of the other chemistries.