Calprotectin Extraction

CALEX Calprotectin Extraction
Fecal Calprotectin Quantification Demands For Enhanced Automation

The manual weighing method with a minimal amount of 50-100 μg fecal sample extracted in 50 volumes of extraction buffer was established in the early 2000 and guarantees the best possible quantification of fCAL. The method is regarded as the reference method for fCAL quantification. New devices simplifying the time consuming reference method have been introduced by many different immunoassay manufacturers. The new CALEX® Cap extraction device from BÜHLMANN was validated in this study.

 Niederberger, C. et al. (2018) Nordic Congress in Clinical Chemistry
Evaluation of BÜHLMANN CALEX® Cap Stool Extraction Devices for the extraction of faecal calprotectin

Calex® extraction devices compare well to extraction via manual weighing. Calex® also demonstrated similar imprecision and accuracy to manual extraction.
The main benefits of Calex®, speed and ease of use, will enable higher throughput and will allow the service to cope with future increases in demand.
The devices are also more hygienic and the significant reduction in staff time and other reagents/consumables will no doubt result in an overall cost saving.

Sukhbir Kaur, Clare Ford, Rousseau Gama – Poster ACB FOCUS 2018
CALEX® Cap, A novel stool collection and extraction device:
Performance Analysis and Stability of Calprotectin in Stool Extracts

The stool sampling using CALEX® Cap is very reproducible, easier and more hygienic than manual weighing. The extraction of
calprotectin from stool samples is as reliable as with conventional methods. CALEX® Cap represents a fully quantitative “all-in-one” device.

 Poster Euromedlab, June 2015
Same faeces, same lab, different methods and some different calprotectin results

Overall the Eurospital extraction devices had more disadvantages than the BÜHLMANN Calex tubes, being more laborious with an acceptable risk of splashing the extract

Lythgoe and McCann;Stockport NHS – Poster BSG 2016
Evaluation of the BÜHLMANN fCAL Turbo Faecal Calprotectin Assay on the Abbott Architect C8000. Comparison to the Phadia EliA and the IBDoc® home test kit

fCAL turbo is very adaptable to the Abbott Architect C8000 analyser. The CALEX ® cap extraction devices are easy to use and practical. Inter-assay variability demonstrated, and recommends users to participate in the UK NEQAS Scheme . No interference to any of the other chemistries.

O’Connell (2017) Research Project Scientific Paper