Calprotectin Extraction

CALEX Calprotectin Extraction
Poo-tentially better? Faecal calprotectin and elastase stability in different sample collection devices and a trial of at home patient-led extraction

Stool analysis for biomarkers such as faecal calprotectin and elastase provides a non-invasive tool to investigate the condition of a patient’s digestive health and help diagnose disease, such as inflammatory bowel disease and pancreatic insufficiency respectively.

Woodley et al Torbay and South Devon NHS Trust
Evaluating the service user experience of CALEX® cap devices as a faecal sample collection method for calprotectin testing: a local audit

Overall, the results affirm that CALEX® cap devices are easy-to-use with accessible instructions and are highly rated over universal tubes by patients. Some invaluable feedback was also obtained, enabling the creation of strategies to improve the service and ensure that faecal sample collection is easy, clear, and comfortable for NHSL service users.

Ralph et al UKMedLab 2023
BÜHLMANN Calex® Cap for the faecal extraction of calprotectin – Fit for purpose?

Calex® devices are fit for purpose, easy to use and offer a quicker extraction process compared with manual weighing and will therefore enable increasing demands of a faecal calprotectin service.

Read the Letter to the Association for Clinical Biochemistry & Laboratory Medicine
How to Prepare an Extraction Control

Preparation of a simple internal quality control for Faecal Calprotectin, from Jane French, Consultant Clinical Scientist, UK NEQAS.

“As Programme Director of the UK NEQAS for Faecal Markers, I am often asked if I can recommend a suitable internal quality control (IQC) material for Faecal Calprotectin. Until quite recently, there has been no commercially-produced IQC available but all is not lost – with a bit of know-how, it is perfectly possible to make your own bespoke IQC, tailored to your exact requirements.”

Read ‘Everything is Under Control!’ from Leading Edge – 2019 Issue 2
Fecal Calprotectin Quantification Demands For Enhanced Automation

The manual weighing method with a minimal amount of 50-100 μg fecal sample extracted in 50 volumes of extraction buffer was established in the early 2000 and guarantees the best possible quantification of fCAL. The method is regarded as the reference method for fCAL quantification. New devices simplifying the time consuming reference method have been introduced by many different immunoassay manufacturers. The new CALEX® Cap extraction device from BÜHLMANN was validated in this study.

 Niederberger, C. et al. (2018) Nordic Congress in Clinical Chemistry
Evaluation of BÜHLMANN CALEX® Cap Stool Extraction Devices for the extraction of faecal calprotectin

Calex® extraction devices compare well to extraction via manual weighing. Calex® also demonstrated similar imprecision and accuracy to manual extraction.
The main benefits of Calex®, speed and ease of use, will enable higher throughput and will allow the service to cope with future increases in demand.
The devices are also more hygienic and the significant reduction in staff time and other reagents/consumables will no doubt result in an overall cost saving.

Sukhbir Kaur, Clare Ford, Rousseau Gama – Poster ACB FOCUS 2018
CALEX® Cap, A novel stool collection and extraction device:Performance Analysis and Stability of Calprotectin in Stool Extracts

The stool sampling using CALEX® Cap is very reproducible, easier and more hygienic than manual weighing. The extraction of
calprotectin from stool samples is as reliable as with conventional methods. CALEX® Cap represents a fully quantitative “all-in-one” device.

Poster Euromedlab, June 2015
Same faeces, same lab, different methods and some different calprotectin results

Overall the Eurospital extraction devices had more disadvantages than the BÜHLMANN Calex tubes, being more laborious with an acceptable risk of splashing the extract

Lythgoe and McCann;Stockport NHS – Poster BSG 2016
Evaluation of the BÜHLMANN fCAL Turbo Faecal Calprotectin Assay on the Abbott Architect C8000. Comparison to the Phadia EliA and the IBDoc® home test kit

fCAL turbo is very adaptable to the Abbott Architect C8000 analyser. The CALEX ® cap extraction devices are easy to use and practical. Inter-assay variability demonstrated, and recommends users to participate in the UK NEQAS Scheme . No interference to any of the other chemistries.

O’Connell (2017) Research Project Scientific Paper